Journal: Ecotoxicology and environmental safety

Article Title: The regulatory effect of pulmonary lymphatic drainage on silicosis fibrosis.

doi: 10.1016/j.ecoenv.2022.113758

Figure Lengend Snippet: Fig. 4. STS promotes lymphangiogenesis through VEGFR-3/PI3K/AKT pathway. (A) The LYVE-1 immunohistochemical (IHC) staining in rat lung. (B) Western blot for VEGFR-3, P-PI3K, and P-AKT expression in the lungs(n = 3 independent experiments). Data are expressed as mean ± SD. *P ≤0.05 compared to control; **P ≤0.01 compared to control; #P ≤0.05 compared to silicosis; ##P ≤0.01 compared to silicosis.

Article Snippet: The following materials and instruments were obtained for this experiment from the denoted manufacturers: SiO2 (particle diameter 0.5–10 μm; Sigma, USA); malondialdehyde (MDA) assay kit(Nanjing Jiancheng Institute of Biological Engineering, China); total antioxidant capacity(T-AOC) assay kit(Nanjing Jiancheng Institute of Biological Engineering, China); laminin (LN) assay kit (Nanjing Jiancheng Institute of Biological Engineering, China); hyaluronic acid(HA) assay kit(Nanjing Jiancheng Institute of Biological Engineering, China);antibody MCP (Abcam, UK); antibody P-AKT (Affinity Biosciences,UK); antibody PPI3K(Affinity Biosciences,UK); lymphatic endothelial hyaluronan receptor 1 (LYVE-1) (sc-19 319, Santa Cruz Biotechnology); antibody VEGFR-3 (Abcam, UK); sodium tanshinone IIA sulfonate(SPH NO.1 Biochemical & Pharmaceutical Ltd, China); RF double pole condenser (Zhangjiagang Aerospace Medical Electrical Appliance Co. Ltd, China); 3M vetbond tissue adhesive(3M, USA);HOPE MED 8050 exposure control apparatus (HOPE Industry and Trade Co. Ltd, Tianjin, China).

Techniques: Immunohistochemical staining, Immunohistochemistry, Western Blot, Expressing, Control

Journal: Frontiers in Physiology

Article Title: Endothelial Microparticle-Mediated Transfer of microRNA-19b Inhibits the Function and Distribution of Lymphatic Vessels in Atherosclerotic Mice

doi: 10.3389/fphys.2022.850298

Figure Lengend Snippet: EMP miR-19b limits lymphatic network distribution along atherosclerotic arteries. (A) Representative immunofluorescence staining images of atherosclerotic arteries from high-fat diet-fed ApoE -/- mice intravenously injected with PBS, EMP control , or EMP miR-19b for 8 weeks. In the images, green fluorescence represents LYVE1, a relatively specific marker of lymphatic vessels, while red fluorescence represents CD68. LYVE1 + /CD68 - tubular structures are identified as lymphatic vessels. (B) Quantitative analysis of the results shown in (A) . * p < 0.05 compared with EMP miR-19b group, n = 7–8 per group.

Article Snippet: The primary antibodies used included lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) mouse monoclonal antibody (Proteintech, United States) and CD68 rabbit polyclonal antibody (Proteintech).

Techniques: Immunofluorescence, Staining, Injection, Control, Fluorescence, Marker